A simplified procedure for large scale preparation of purified animal DNA-dependent RNA polymerase.
نویسندگان
چکیده
A procedure is described for obtaining DNA-dependent RNA polymerase form B from calf thymus in high purity. The procedure includes homogenization in low salt, sedimentation of chromatin at 50 000 x g, adsorption to DEAE-cellulose in a batch procedure, DNA-agarose chromato graphy, and chromatography on DEAE-Sephadex. Analyses of the preparation by band sedimenta tion and gel-electrophoresis indicate a high purity of the enzyme.
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ورودعنوان ژورنال:
- Zeitschrift fur Naturforschung. Teil C: Biochemie, Biophysik, Biologie, Virologie
دوره 28 9 شماره
صفحات -
تاریخ انتشار 1973